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Compared to untreated normal urothelium, decreased amounts of H3K4 mono-methylation, up-regulation of potential H3K4 target genes (i.e. HOX C8, 11, 13 and D1 ), and down-regulation of sFRP1 gene were seen in BCa tissues of mice treated with OH-BBN; these effects of OH-BBN were all reversed by kawain treatment. Treatment of NHBEC with 4-ABP also decreased the levels of H3K4 methylation, whereas kawain induced H3K4 mono- and di-methylation in BCa cells.

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